The following essay is reprinted with permission. Talkonline publication dedicated to the latest research.
Human DNA can be sequenced from small amounts of water, sand and air in the environment to potentially extract identifiable information like genetic background, gender and health risks, according to our new study.
Every cell of the body contains DNA. Because each person has a unique genetic code, DNA can be used to identify individuals. Typically, practitioners and researchers obtain human DNA through direct sampling, such as blood tests, swabs, or biopsies. However, all living beings, including animals, plants and microbes, constantly shedding DNA. Water, soil and even air contain microscopic particles of the biological material of living organisms.
The DNA that an organism releases into the environment is called ecological DNA or eDNA. Over the past couple of decades, scientists have been able to collect and sequence eDNA from soil or water samples in monitoring of biodiversity, populations of wild animals another pathogens. Tracking rare or elusive endangered species through their eDNA has been a boon to researchers, as traditional monitoring methods such as observation or trapping can be difficult, often unsuccessful, and intrusive to the species of interest.
Researchers using eDNA tools typically only focus on the species they are studying and ignore the DNA of other species. However, people so shedcough and flush DNA into their environment. And as our team of geneticists says, ecologists another marine biologists V Duffy’s lab found at the University of Florida, signs of human life can be found everywhere but in the most isolated places.
Animals, humans and viruses in eDNA
Our team uses environmental DNA to study endangered sea turtles and viral tumors to which they are subject. Tiny hatchling sea turtles lose their DNA as they crawl along the shore on their way to the ocean shortly after birth. Sand scooped from their footprints contains enough DNA to provide valuable information about tortoises and chelonid herpesviruses and fibropapillomatous tumors affecting them. Scoop up a liter tank water a recovering sea turtle under veterinary care equally provides a wealth of genetic information for research. Unlike blood or skin samples, eDNA collection does not stress the animal.
Genetic sequencing technology The methods used to decipher DNA have improved rapidly in recent years and it is now possible to easily sequence the DNA of each organism in an environmental sample. Our team suspected that the sand and water samples we used to study sea turtles might also contain DNA from a number of other species, including, of course, humans. that we didn’t know that how informative the human DNA we could extract would be like this.
To find out, we took samples from a variety of places in Florida, including the ocean and rivers in urban and rural areas, sand from isolated beaches, and a remote island not normally visited by humans. We found human DNA in all of these locations, except for the remote island, and these samples were of high enough quality for analysis and sequencing.
We also tried this technique in Ireland, following a river that flows from a remote mountaintop, through small rural villages, and into the sea in a larger city of 13,000 people. We have found human DNA everywhere except in a remote mountain tributary where a river flows, away from human habitation.
We also took air samples from a room at our Florida Wildlife Veterinary Hospital. The people present in the room allowed us to take air samples. We recovered DNA matching DNA from humans, the animal patient, and common animal viruses present at the time of collection.
Surprisingly, the human eDNA found in the local environment was intact enough for us to be able to identify mutations associated with the disease and determine the genetic origins of people living in the area. DNA sequencing, left by volunteers in the form of footprints in the sand, even made it possible to identify part of their sex chromosomes.
Ethical implications of human eDNA collection
Our team duplicates the unintentional extraction of human DNA from environmental samples “Human genetics by catch”. We call for a deeper discussion on how to ethically handle the human DNA of the environment.
Human EDNA could provide significant advances in research in areas as diverse as conservation, epidemiology, forensics, and agriculture. If handled correctly, human eDNA could help archaeologists track down unexplored ancient human settlementsallow biologists Monitoring cancer mutations in a given population or provide law enforcement useful forensic information.
However, there are many ethical implications associated with the unintentional or intentional collection and analysis of human genetic by-catch. Identifiable information can be retrieved from eDNA and access to this level of detail on individuals or populations is responsibilities related to consent and confidentiality.
Although we conducted our study with the approval of our institutional review boardwhich guarantees that human research complies with the ethical principles of research, there is no guarantee that everyone will treat this type of information ethically.
Many questions arise regarding the human DNA of the environment. For example, who should have access to human eDNA sequences? Should this information be publicly available? Is consent required before human eDNA sampling and from whom? Should researchers remove human genetic information from samples originally collected to identify other species?
We believe it is critical to put in place policies that ensure that data is collected, analyzed and stored ethically and appropriately. Policy makers, the scientific community, and other stakeholders must take the collection of human eDNA seriously and balance consent and privacy with the possible benefits of studying eDNA. Raising these questions now can help ensure that everyone is aware of the potential of eDNA and allow more time to develop protocols and regulations to ensure the proper use of eDNA techniques and the ethical management of human genetic by-catch.
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